Gant A, Leyva VE, Gonzalez AE, Maruenda H.  J AOAC Int, 98(1):98-102. doi:10.5740/jaoacint.14-113

This paper describes a rapid and validated HPLC-diode array detector method for the simultaneous quantitation of caffeine, trigonelline, nicotinic acid, N-methylpyridinium ion,5-caffeoylquinic acid, and 5-hydroxymethyl furfural that is applicable to three coffee matrixes: green, roasted, and instant. The method showed good linear correlation (r² > 0.9985), precision (less than 3.9%), sensitivity (LOD = 0.023–0.237 μg/mL; LOQ = 0.069–0.711 μg/mL), and recovery (84–102%)for all compounds. This simplified method is amenable for a more complete routine evaluation of coffee in industry.